Hvita 3D Perfusion Organoid Culture System

Key Features

  • Automated organoid culture eliminates frequent manual medium changes and reduces contamination risk.
  • Programmable perfusion culture enables reproducible organoid production.
  • Compact incubator provides stable culture environment on lab benchtop.
  • Open-chamber design is compatible with various organ-on-a-chip devices

Achieving high-quality and high-yield production of organoids

The integrated perfusion provides efficient medium
exchange without disturbing Matrigel, resulting in
more homogenous organoid growth with higher
viability rate than traditional plate culture.

  • Viability rate was observed to be 1.3-15 folds
    higher for a variety of organoids.
  • The Hvita system also supports high seeding
    density up to 600 organoids per 100μL

Hvita                  24-Well Plate

Human Gastric Cancer Organoids

Human Liver Organoids

Mouse Lung Organoids

Simplifying operation for process standardization

  • Eliminates manual media exchange through embedded perfusion system
  • Reduces operator manual tasks.
  • Facilitates process standardization to ensure consistent quality at industry scale.
Incubator CO2 concentration range/accuracy0-20% / ±0.1%
Incubator temperature range/accuracy34-40ºC / ±0.3ºC
Perfusion rate/accuracy1μL/min - 6 mL/min / ±10%
Number of perfusion pumps per incubator6
Number of incubators supported per controllerUp to 4
Organoid factory – culture volume1mL - 3mL
Organoid factory – medium volume4.5mL- 35mL
Organoid factory – membrane pore size0.8μm
Incubator dimensions15.4 x9.1x2.6 inch (39x23x6.5 cm)
Incubator net weight8.2 lbs. (3.7kg)
Controller dimensions11 x14.6 x9.5 inch (28x37x24 cm)
Controller net weight12.3 lbs. (5.6kg)
Power requirement110-240V/AC, 50/60Hz, 240W

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